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• In the first group , where WT peptides had been poor or null binders, an necessary proportion of peptides contained the mutation at HLA-anchoring residues (e.g. peptides CAM69-6, CAM73-9).
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Prediction of binding to HLA class I molecules of mutated and wild type peptides. Finally, to show recognition of processed antigens in the context of tumor cells, we prepared transfectants of MC703 cells expressing the neoAgs used for vaccination. We demonstrated that T cells induced by DNA vaccines, mainly for these corresponding to affected person CAM69, particularly acknowledged tumor cells expressing the neoAgs but not control cells . As shown in Figure 5D, these T cells particularly acknowledged neoAg-expressing tumor cells. NeoAg-specific responses had been evaluated at day 21 of co-culture through the use of a human IFN-γ ELISPOT set (BD-Biosciences) following manufacturer’s directions.

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Different criteria have been used to pick predicted peptides. In our case, along with a binding threshold for all mutated peptides, we distinguished two groups relating to the binding capacity of the WT peptide. In the first group , the place WT peptides were poor or null binders, an essential proportion of peptides contained the mutation at HLA-anchoring residues (e.g. peptides CAM69-6, CAM73-9). However, within the second group all peptides contained the mutation at non-anchor residues.

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